Immunoprecipitation was performed on HEK293T cells overexpressing LGR5. Antigen-antibody complexes were formed by incubating the sample with 2 µg of LGR5 monoclonal antibody (Product # MA5-25644) antibody and 0.1 mg of goat anti-mouse conjugated magnetic beads overnight. LGR5 (GPR49) Antibody (loop1) Purified Rabbit Polyclonal Antibody (Pab) Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (loop1) (Cat.#AP2745c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Product Information. Product nameLGR5 Polyclonal Antibody. ImmunogenSynthesized peptide derived from part region of human LGR5 protein. HostRabbit. ReactivityHuman, Mouse.
![Lgr5 Antibody Immunofluorescence Lgr5 Antibody Immunofluorescence](/uploads/1/2/5/6/125602309/631980962.jpg)
ApplicationsELISA, WB. Application notesOptimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000). ClonalityPolyclonal. IsotypeRabbit IgG. PurificationThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogenProduct Properties. FormulationLiquid solution.
Concentration1 mg/ml. Storage bufferPBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
![Lgr5 antibody ihc Lgr5 antibody ihc](https://www.researchgate.net/profile/Christine_Boeger/publication/224822174/figure/fig10/AS:339850285862922@1458038031188/LGR5-immunoreactivity-in-hepato-gastrointestinal-tissues-Expression-of-LGR5-in-normal_Q320.jpg)
Storage instructionsStable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. ShippingGel pack with blue ice.
PrecautionsThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.Additional Information. BackgroundThe protein encoded by LGR5 is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development.
Several transcript variants encoding different isoforms have been found for LGR5. Gene ID. Alternative namesLeucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38. OthersThe antibody detects endogenous levels of LGR5 protein. Accession.
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.Preparation methods for BiochemicalBiochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay.
However, some of tips and suggestions listed below may be for your reference.Antibody application protocolsAntibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.Protein&peptide usage suggestionsSynthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.Commonly used assay kits guidelinesAssay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.We hope this will be helpful for your research work. Please let us know through if you need more information or support.
Secondary Antibodies Available DescriptionProduct CodeApplicationsPack SizeYour PriceList PriceQuantity2AB02BCEPWB1 mlAdd to Cart Add to QuoteSTAR121BEWB1 mgAdd to Cart Add to QuoteSTAR121FF1 mgAdd to Cart Add to QuoteSTAR121PEWB1 mgAdd to Cart Add to QuoteSTAR124PCEWB1 mgAdd to Cart Add to QuoteSTAR34BCF1 mgAdd to Cart Add to QuoteSTAR35AF1 mlAdd to Cart Add to QuoteSTAR36D488GAFIFWB0.1 mgAdd to Cart Add to QuoteSTAR36D680GAFIFWB0.1 mgAdd to Cart Add to QuoteSTAR36D800GAFIFWB0.1 mgAdd to Cart Add to Quote. Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping cartsEdit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other cartsInspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents.